Klk, i used to take mine always aftr 17h00 afternoons, now i'm using it round about 12h00 midday and drink not much during the morning so its still concentrated. My first one in Dec after several mths of negatives i got at 12h40 and again at 17h00. This mth i started at cd10 and got my positive first time, so i assume O occured on cd11. On the paper inside it told you when to start testing. Sometime i test 2x /day between cd11 and 15. Hope it helps!! Good luck!